Question

Difficulty: EasyDetermining Control Groups and Baseline Conditions

A researcher investigated the effect of different concentrations of glucose on the fermentation rate of yeast (*Saccharomyces cerevisiae*). Five test tubes were prepared, each containing 10 mL10\text{ mL} of a yeast suspension and a specific concentration of glucose dissolved in distilled water. The tubes were maintained at 30C30^\circ\text{C} for 3030 minutes. The volume of carbon dioxide (CO2\text{CO}_2) gas produced in each tube was measured to determine the rate of fermentation. The experimental setups are shown in the table below:

TubeYeast Suspension (mL\text{mL})Glucose Concentration (%\%)Distilled Water (mL\text{mL})Temperature (C^\circ\text{C})
11001030
21021030
31051030
410101030
510201030

Which of the following tubes served as the control group to establish the baseline level of gas production in the absence of glucose?

  1. Tube 1, because it contains 0%0\% glucose, allowing the researcher to measure gas production in the absence of the independent variable.Answer
  2. B
    Tube 3, because it contains a moderate glucose concentration of 5%5\% to represent typical environmental conditions.
  3. C
    Tube 5, because it contains the highest glucose concentration of 20%20\% to show the maximum possible fermentation rate.
  4. D
    None of the tubes, because a true control group would require a tube with glucose and water but no yeast suspension.

Answer

Tube 1, because it contains 0%0\% glucose, allowing the researcher to measure gas production in the absence of the independent variable.
The correct answer identifies the tube containing 0%0\% glucose. In an experiment, the control group is the baseline condition where the independent variable (in this case, glucose concentration) is absent, allowing researchers to isolate the effects of the independent variable.

Step-by-Step Solution

1
Identify the independent variable being manipulated.
The independent variable is the concentration of glucose, which varies from 0%0\% to 20%20\%.
The control group requires the independent variable to be absent or set to a baseline level.
2
Identify the tube where this independent variable is absent.
Tube 1 contains 0%0\% glucose.
This establishes the baseline activity of the yeast in the absence of the added sugar.
3
Verify that all other variables remain constant.
Tube 1 contains the same volume of yeast suspension (10 mL10\text{ mL}), distilled water (10 mL10\text{ mL}), and is kept at the same temperature (30C30^\circ\text{C}) as all other groups.
A valid control group must keep all controlled variables identical to the experimental groups.

Key Concept

Determining Control Groups and Baseline Conditions
Estimated Time:1m 0s
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