Bioluminescence in dinoflagellates—single-celled marine organisms—is a defensive mechanism triggered by mechanical stress, such as the shear forces of a breaking wave or a passing predator. When the cell membrane of a dinoflagellate is physically deformed by movement in the surrounding water, it initiates an action potential that propagates across the vacuole membrane. This electrical signal stimulates the opening of voltage-gated proton channels, which allows protons to flood from the acidic vacuole into the scintillon, a specialized cytoplasmic pocket. The resulting decrease in pH within the scintillon activates the enzyme luciferase. Once activated, luciferase catalyzes the oxidation of luciferin, a light-emitting pigment. This rapid chemical reaction produces a flash of blue-green light that lasts for about 0.1 seconds. Ecologists hypothesize that this sudden bioluminescent display startles predators, effectively disrupting their feeding behavior and allowing the dinoflagellate to escape unharmed.
According to the passage, the activation of the enzyme luciferase is directly caused by which of the following?
- Aan increase in pH within the acidic vacuole
- a decrease in pH within the scintillonAnswer
- Cthe physical deformation of the vacuole membrane
- Dthe oxidation of the light-emitting pigment luciferin