To investigate the effect of wind speed on the rate of transpiration, students placed four identical oak saplings in potometers. Each sapling was exposed to a fan at a different distance to vary the wind speed, with Trial 4 serving as the control group (no fan). The trials were conducted sequentially inside a greenhouse, and the ambient temperature, relative humidity, and transpiration rate were recorded for each 1-hour trial. The results are shown in the table below.
| Trial | Distance from fan () | Time of day | Temperature () | Relative Humidity () | Transpiration rate () |
|---|---|---|---|---|---|
| 1 | 10:00 AM – 11:00 AM | ||||
| 2 | 11:00 AM – 12:00 PM | ||||
| 3 | 12:00 PM – 1:00 PM | ||||
| 4 | No fan | 1:00 PM – 2:00 PM |
Based on these results, the students concluded that a wind speed corresponding to a distance of from the fan maximizes the rate of transpiration in oak saplings. Which of the following statements identifies the primary flaw in this conclusion?
- AThe students failed to control the dependent variable (distance from the fan), which should have been kept constant while the independent variable (transpiration rate) was measured.
- The sequential execution of the trials allowed ambient temperature to rise and relative humidity to fall over time, both of which independently increase transpiration rates and obscure the true effect of wind speed.Answer
- CTrial 4 cannot serve as a control group because a true control group requires the fan to be set to a baseline low speed rather than being shut off completely.
- DBecause transpiration rate is inversely proportional to temperature, the higher temperature in Trial 3 should have decreased transpiration, proving that wind speed was the sole driver of the observed increase.
Answer
The correct answer states that the sequential execution of the trials allowed ambient temperature to rise and relative humidity to fall over time, both of which independently increase transpiration rates and obscure the true effect of wind speed.
The correct option is correct because conducting the trials sequentially throughout the day allowed ambient temperature to rise and relative humidity to fall. Both higher temperatures and lower relative humidity increase the rate of transpiration by increasing the rate of water evaporation from the leaves. Because these environmental factors changed systematically alongside the distance from the fan, they act as confounding variables, making it impossible to determine whether the differences in transpiration rate were caused by the change in wind speed or the changes in temperature and humidity.
Step-by-Step Solution
Key Concept
Identifying Confounding Variables
Estimated Time:2m 30s