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Difficulty: MediumSequential Detail Extraction

During field biological research, preserving tissue samples in liquid nitrogen requires a specific cryopreservation routine to prevent cellular crystallization. First, the field biologist extracts the specimen and immerses it in a chemical cryoprotectant solution. After the tissue absorbs the solution for ten minutes, excess fluid is blotted using sterile filter paper. Next, the specimen is sealed inside an airtight cryovial before being placed into an isopropyl alcohol cooling container to gradually lower its temperature. Only after this controlled cooling phase reaches minus eighty degrees Celsius is the vial transferred to the liquid nitrogen storage dewar for long-term preservation.

According to the passage, which action must be performed immediately after blotting excess fluid from the specimen?

  1. A
    Placing the specimen into an isopropyl alcohol cooling container
  2. B
    Immersing the extracted tissue in a chemical cryoprotectant solution
  3. Sealing the specimen inside an airtight cryovialAnswer
  4. D
    Transferring the vial to the liquid nitrogen storage dewar

Answer

Sealing the specimen inside an airtight cryovial
The passage states that after excess fluid is blotted using sterile filter paper, the immediate next action is that 'the specimen is sealed inside an airtight cryovial.' Therefore, sealing the cryovial directly follows blotting.

Step-by-Step Solution

1
Locate the reference step mentioned in the question stem within the passage.
The reference step is 'excess fluid is blotted using sterile filter paper.'
Identifying the target milestone establishes the correct chronological starting point.
2
Analyze the sequential transition indicators that immediately follow the blotting step.
The passage introduces the subsequent action with the transition word 'Next' followed by 'the specimen is sealed inside an airtight cryovial.'
Tracking chronological sequence markers identifies the direct precursor-to-subsequent relationship.
3
Differentiate the immediate next step from subsequent secondary actions.
Sealing the cryovial occurs before placing the vial in the cooling container and long before transfer to the liquid nitrogen storage dewar.
Ensures that intermediate procedural steps are not bypassed.

Key Concept

Sequential Detail Extraction
Estimated Time:1m 0s
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Topics

Sequential Detail Extraction

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