Question

Difficulty: MediumViruses: Structure and Characteristics

An isolated biological agent undergoes crystallization when stored in a sterile nutrient broth outside host cells. However, upon entry into a susceptible living host cell, it initiates replication. Which of the following best explains why this agent is incapable of independent metabolic activity outside a host?

  1. It lacks cellular structures such as cytoplasm, ribosomes, and metabolic enzymes necessary for self-sustained protein synthesis.Answer
  2. B
    Its cytoplasm is enclosed by a semi-permeable cell membrane that becomes inactive when removed from host tissue.
  3. C
    Its membrane-bound organelles enter a dormant state outside host cells due to a lack of cellular ATP reserves.
  4. D
    Its peptidoglycan cell wall thickens in extracellular fluid, preventing the absorption of nutrient molecules.

Answer

It lacks cellular structures such as cytoplasm, ribosomes, and metabolic enzymes necessary for self-sustained protein synthesis.
The correct option correctly identifies that viruses are acellular entities lacking cytoplasm, ribosomes, and metabolic enzymes, rendering them incapable of self-sustained protein synthesis or metabolism outside a living host cell.

Step-by-Step Solution

1
Analyze the structural organization of viruses.
Viruses are acellular entities composed primarily of genetic material (DNA or RNA) contained within a protein coat (capsid).
Establishing acellular status explains why viral particles display non-living characteristics (such as crystallization) when isolated outside host organisms.
2
Determine the biochemical requirements for independent metabolic activity.
Independent protein synthesis and ATP generation require cytoplasm, functional ribosomes, and metabolic enzymes—all of which viruses lack.
Because viruses lack this cellular machinery, they act as obligate intracellular parasites that must hijack host cellular systems to replicate.

Key Concept

Acellular nature and obligate intracellular parasitism of viruses
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