Question

Difficulty: EasyReduction of Risk Potential

A nurse is preparing to obtain a sterile urine specimen for culture and sensitivity from a client who has a continuous indwelling urinary catheter. Arrange the following steps in the correct order to minimize the risk of infection and ensure an accurate sample.

  1. 1Clamp the drainage tubing below the aspiration port
  2. 2Cleanse the aspiration port with an antiseptic wipe
  3. 3Attach a sterile syringe and withdraw the urine sample
  4. 4Transfer the urine into a sterile specimen container
  5. 5Unclamp the drainage tubing

Answer

The correct sequence is: Clamp the drainage tubing, cleanse the aspiration port, attach a syringe to aspirate urine, transfer the urine to a sterile container, and finally unclamp the drainage tubing.
To obtain a sterile urine specimen from a closed indwelling catheter system, the nurse must first clamp the tubing below the port to allow fresh urine to collect. Next, the port must be cleansed with an antiseptic to maintain sterility. The nurse then uses a sterile syringe to aspirate the urine, transfers it to a sterile container, and finally unclamps the tubing to allow continuous drainage to resume.

Step-by-Step Solution

1
Clamp the drainage tubing below the aspiration port
Fresh urine accumulates in the tubing above the clamp.
Urine in the drainage bag is not sterile and may have been sitting for hours. Clamping ensures a fresh sample is collected from the bladder.
2
Cleanse the aspiration port with an antiseptic wipe
The surface of the port is disinfected.
This prevents the introduction of microorganisms from the port surface into the closed drainage system.
3
Attach a sterile syringe and withdraw the urine sample
A sterile sample of fresh urine is obtained.
Using a sterile syringe maintains the sterility of both the closed catheter system and the urine specimen.
4
Transfer the urine into a sterile specimen container
The sample is secured for laboratory transport.
A sterile container is required for a culture and sensitivity test to accurately identify any urinary pathogens.
5
Unclamp the drainage tubing
Continuous urinary drainage is restored.
Failing to unclamp the tubing can cause bladder distention, discomfort, and potentially harmful retrograde flow of urine.

Key Concept

Maintaining a sterile closed drainage system when obtaining a urine culture from an indwelling catheter reduces the risk of catheter-associated urinary tract infections (CAUTI) and ensures diagnostic accuracy.
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