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Difficulty: EasyBiotechnology and Nanotechnology

With reference to modern biotechnology, consider the following statements regarding CRISPR-Cas9 technology:

1. It functions as a precise genome-editing tool in living organisms.
2. The Cas9 enzyme acts as molecular scissors to cut target DNA sequences.

Which of the statements given above is/are correct?

  1. A
    1 only
  2. B
    2 only
  3. Both 1 and 2Answer
  4. D
    Neither 1 nor 2

Answer

Both statements 1 and 2 are correct.
Both statements are correct. CRISPR-Cas9 is a major biotechnological advancement adapted from bacterial immune systems. Guide RNA leads the complex to a complementary target sequence on the DNA strand, and the Cas9 enzyme acts as molecular scissors to cut the DNA, enabling precise genomic modifications.

Step-by-Step Solution

1
Evaluate Statement 1 regarding the application of CRISPR-Cas9.
Statement 1 is correct because CRISPR-Cas9 is widely used as a gene-editing technology to add, remove, or alter genetic material in organisms.
CRISPR enables precise modification of DNA sequences within genomes.
2
Evaluate Statement 2 regarding the specific role of the Cas9 component.
Statement 2 is correct because Cas9 is an endonuclease protein that cuts double-stranded DNA at targeted sites specified by guide RNA.
The endonuclease activity of Cas9 functions analogously to molecular scissors.

Key Concept

CRISPR-Cas9 Genome Editing Mechanism
Estimated Time:45s
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Topics

Biotechnology and Nanotechnology

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