A team of biochemists investigated the catalytic activity of the enzyme \beta-glucosidase … — ACT

A team of biochemists investigated the catalytic activity of the enzyme $\beta$ -glucosidase by measuring the rate of cellobiose hydrolysis across five different incubation temperatures ( $20^\circ\text{C}$ , $30^\circ\text{C}$ , $40^\circ\text{C}$ , $50^\circ\text{C}$ , and $60^\circ\text{C}$ ). In each trial, the buffer solution pH ( $6.0$ ), cellobiose concentration ( $10\text{ mM}$ ), and total enzyme volume ( $0.5\text{ mL}$ ) were held constant. The rate of glucose production ( $\mu\text{mol/min}$ ) was recorded after a 10-minute reaction period.

Which of the following correctly identifies the independent variable and the dependent variable in this experiment?

A
The independent variable is the rate of glucose production, and the dependent variable is the incubation temperature.
The independent variable is the incubation temperature, and the dependent variable is the rate of glucose production.Cevap
C
The independent variable is the incubation temperature, and the dependent variable is the pH of the buffer solution.
D
The independent variable is the enzyme volume, and the dependent variable is the rate of glucose production.

Cevap

The independent variable is the incubation temperature, and the dependent variable is the rate of glucose production.

The independent variable is the condition directly altered by the researchers across experimental trials (

20^\circ\text{C}, 30^\circ\text{C}, 40^\circ\text{C}, 50^\circ\text{C}

, and

60^\circ\text{C}

). The dependent variable is the resulting quantitative measurement recorded by the researchers (rate of glucose production in

\mu\text{mol/min}

). All other parameters, such as buffer pH, substrate concentration, and enzyme volume, are controlled variables kept constant across all groups.

Adım Adım Çözüm

Identify the variable directly manipulated by the experimenters.

The researchers set five distinct temperature conditions (

20^\circ\text{C}

60^\circ\text{C}

). Therefore, incubation temperature is the independent variable.

The independent variable is the factor systematically varied to test its effect.

Identify the variable measured as an outcome of the manipulation.

The rate of glucose production was measured after 10 minutes. Therefore, the rate of glucose production is the dependent variable.

The dependent variable is the observed response that changes depending on the independent variable.

Distinguish experimental variables from controlled conditions.

Factors such as buffer pH (

6.0

), cellobiose concentration (

10\text{ mM}

), and enzyme volume (

0.5\text{ mL}

) were maintained at fixed values across all test runs.

Controlled variables must remain constant across all trials to isolate the specific impact of the independent variable.

Anahtar Kavram

Identifying Independent, Dependent, and Controlled Variables

Soru

Cevap

Adım Adım Çözüm

Anahtar Kavram

Sık Yapılan Hatalar

Konular

Bu konudan daha fazla soru