Botanists conducted an experiment to evaluate how varying concentrations of ground-level ozone () affect the stomatal conductance of soybean (*Glycine max*) leaves over a 14-day period. Four identical growth chambers were maintained at constant temperature (), relative humidity (), and photosynthetic photon flux density. The chambers received the following gaseous treatments:
- Chamber 1: Charcoal-filtered air containing of .
- Chamber 2: Purified air containing of .
- Chamber 3: Purified air containing of .
- Chamber 4: Purified air containing of .
Which chamber served as the negative control group in this experiment, and what baseline condition did it establish?
- Chamber 1; it established baseline stomatal conductance in the complete absence of ozone exposure.Cevap
- BChamber 2; it established baseline stomatal conductance under minimal ozone exposure.
- CChamber 4; it established the baseline maximum stress response of the soybean plants.
- DChambers 2, 3, and 4 combined; they established the average baseline across all ozone concentrations.
Cevap
Chamber 1 served as the control group, establishing baseline stomatal conductance in the absence of ozone exposure.
In experimental design, a negative control group establishes a benchmark standard by eliminating the independent variable. Chamber 1 receives charcoal-filtered air with of , allowing researchers to measure normal, unexposed stomatal conductance and confirm that any changes in Chambers 2–4 are caused by ozone exposure.
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Determining Control Groups and Baseline Conditions