Passage
In the late summer of 1928, bacteriologist Alexander Fleming left his laboratory at St. Mary's Hospital in London for a family vacation. Before departing, he stacked several petri dishes containing cultures of *Staphylococcus* bacteria on a bench in the corner of his room. During his absence, a window was left open, allowing spores of a rare mold, *Penicillium notatum*, to drift in from a laboratory downstairs. The mold spores landed on one of the exposed culture plates and began to grow. Upon returning in September, Fleming noticed a strange phenomenon: the bacterial colonies immediately adjacent to the growing mold had dissolved, leaving a distinct, clear halo of lysis. Fleming correctly hypothesized that the mold was secreting a self-defense substance that actively destroyed the *Staphylococcus* cell walls. This substance, which he named penicillin, went on to revolutionize medicine.
Based on the passage, in what chronological order did the events leading to the identification of penicillin occur?
- 1Fleming leaves stacked bacterial cultures unattended on a laboratory bench.
- 2Spores of the mold *Penicillium notatum* contaminate a stacked culture plate of *Staphylococcus* bacteria.
- 3The *Staphylococcus* bacterial cells near the growing mold are lysed and dissolve.
- 4Fleming notices a clear halo around the mold and concludes that it produces an antibacterial agent.