Agricultural Scientist: In a field trial across thirty commercial apple orchards, trees treated with a foliar spray containing live *Bacillus* bacteria produced fruit with significantly lower rates of bitter rot fungal infection than untreated trees did. Because chemical analyses revealed that the spray did not alter the thickness or acidity of the apple skin, the scientist concluded that the *Bacillus* bacteria themselves actively suppress the germination of bitter rot fungal spores on the fruit's surface.
Which of the following, if true, most strengthens the agricultural scientist's argument?
- AIn laboratory assays, bitter rot fungal spores germinate at identical rates regardless of whether live *Bacillus* bacteria are present in the culture medium.
- BCommercial apple growers who do not use biological bacterial sprays typically rely on synthetic chemical fungicides to manage fungal outbreaks.
- Trees sprayed with a sterile solution identical to the foliar spray in every respect except lacking live *Bacillus* bacteria experienced rates of bitter rot infection comparable to those of untreated trees.Cevap
- DThe specific strain of *Bacillus* bacteria utilized in the trial is the single most effective biological control agent ever documented against agricultural pathogens.
- ECommercial apple orchards situated in humid regional climates generally exhibit higher baseline frequencies of bitter rot infection than those in arid climates.
Cevap
The statement showing that trees sprayed with a sterile solution containing all spray ingredients except the live bacteria suffered infection rates comparable to untreated trees.
The argument concludes that live *Bacillus* bacteria were the direct cause of the lower infection rates. A major vulnerability of this inference is that the liquid spray itself or other inactive carrier ingredients might have washed away spores or created a hostile environment. By showing that an identical spray without live bacteria yields infection rates no better than no treatment at all, the correct choice acts as a negative control, ruling out the carrier solution as an alternative cause and strongly reinforcing the causal role of the bacteria.
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Causal strengthening via ruling out confounding variables and establishing negative controls
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