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Zorluk: ZorReduction of Risk Potential

A licensed practical nurse (LPN) is preparing to collect an aerobic wound culture from a client's sacral pressure injury. To ensure the integrity of the diagnostic test and reduce the risk of inaccurate laboratory results, in which order should the nurse perform the following actions? (Arrange the steps in the correct chronological sequence.)

  1. 1Remove the existing soiled dressing and assess the appearance of the wound bed.
  2. 2Irrigate the wound thoroughly with sterile normal saline.
  3. 3Rotate a sterile culture swab over an area of viable, granulating tissue.
  4. 4Insert the swab directly into the sterile collection tube without touching the outer rim.
  5. 5Apply a new prescribed sterile dressing and document the procedure.

Cevap

The correct sequence requires exposing the wound, cleansing it with sterile saline, swabbing the viable tissue, securing the specimen, and finally redressing the wound.
The correct sequence ensures that the laboratory culture identifies the specific pathogen actively causing the tissue infection, rather than identifying normal surface skin flora or dead organisms found in surface exudate. Therefore, the wound must always be irrigated with sterile normal saline BEFORE the swab is applied to viable, granulating tissue. Proper sequencing reduces the risk of misdiagnosis and inappropriate antibiotic therapy.

Adım Adım Çözüm

1
Remove the dressing and assess the wound.
The wound is exposed for baseline evaluation.
Allows the nurse to observe the site and identify the best area for specimen collection.
2
Irrigate with sterile normal saline.
Surface exudate and debris are washed away.
Cleansing prevents normal resident skin flora or dead neutrophils (pus) from contaminating the sample and causing inaccurate lab results.
3
Swab viable granulating tissue.
A clean pathogen sample is obtained.
Actively replicating microorganisms causing the deep tissue infection reside in the healthy-looking, granulating tissue.
4
Insert the swab into the sterile transport tube.
The specimen is secured and preserved.
Maintains a sterile environment and prevents external contamination during transport to the laboratory.
5
Apply a fresh dressing and document.
The wound is protected and the medical record is updated.
Completes the procedure by re-establishing a protective barrier over the injury.

Anahtar Kavram

Diagnostic test preparation: Aerobic wound culture collection technique
Tahmini Süre:1m 30s
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