Question

Difficulty: MediumFormulating and Modifying Hypotheses

A student conducted an experiment to investigate the effect of light wavelength on the rate of photosynthesis in *Elodea* plants. The student formulated the following hypothesis:

*Hypothesis:* The rate of photosynthesis, as measured by the volume of oxygen (O2O_2) gas produced per hour, increases continuously as the wavelength of light increases from 400 nm400\text{ nm} to 700 nm700\text{ nm}.

The student exposed identical *Elodea* plants to different wavelengths of light for 1 hour each, keeping all other environmental variables constant. The results are shown in the table below:

WavelengthVolume of O2O_2 produced
400 nm400\text{ nm}1.2 mL1.2\text{ mL}
450 nm450\text{ nm}4.5 mL4.5\text{ mL}
550 nm550\text{ nm}0.3 mL0.3\text{ mL}
650 nm650\text{ nm}5.8 mL5.8\text{ mL}
700 nm700\text{ nm}1.0 mL1.0\text{ mL}

Based on the results of the experiment, does the data support the student's hypothesis, and how should the hypothesis be modified?

  1. A
    Yes; the data show that the overall rate of photosynthesis increases continuously from 400 nm400\text{ nm} to 700 nm700\text{ nm}.
  2. No; the rate of photosynthesis does not increase continuously, so the hypothesis should be modified to state that photosynthesis peaks under blue (450 nm450\text{ nm}) and red (650 nm650\text{ nm}) light.Answer
  3. C
    Yes; the rate of photosynthesis is highest at 650 nm650\text{ nm}, which is greater than the rate at any lower wavelength tested.
  4. D
    No; the rate of photosynthesis decreases continuously, so the hypothesis should be modified to state that the rate is inversely proportional to wavelength.

Answer

No; the rate of photosynthesis does not increase continuously, so the hypothesis should be modified to state that photosynthesis peaks under blue (450 nm450\text{ nm}) and red (650 nm650\text{ nm}) light.
The correct answer is correct because the experimental results show that oxygen production does not increase continuously. Instead, it rises and falls, reaching local maxima at 450 nm450\text{ nm} and 650 nm650\text{ nm}, and a minimum at 550 nm550\text{ nm}. Therefore, the hypothesis is unsupported and must be modified to state that photosynthesis is most efficient (peaks) at specific wavelengths corresponding to blue and red light.

Step-by-Step Solution

1
Analyze the student's hypothesis.
The hypothesis predicts a continuous, monotonic increase in oxygen production as wavelength increases from 400 nm400\text{ nm} to 700 nm700\text{ nm}.
Understanding the prediction is necessary to evaluate it against the experimental data.
2
Examine the trend in the data table.
The volume of oxygen increases from 1.2 mL1.2\text{ mL} (at 400 nm400\text{ nm}) to 4.5 mL4.5\text{ mL} (at 450 nm450\text{ nm}), drops to 0.3 mL0.3\text{ mL} (at 550 nm550\text{ nm}), increases to 5.8 mL5.8\text{ mL} (at 650 nm650\text{ nm}), and drops again to 1.0 mL1.0\text{ mL} (at 700 nm700\text{ nm}).
This determines whether the actual rate of photosynthesis increases continuously.
3
Compare the data to the hypothesis and determine the appropriate modification.
Because the trend shows peaks at 450 nm450\text{ nm} and 650 nm650\text{ nm} rather than a continuous increase, the hypothesis is not supported and should be modified to describe these dual peaks.
Formulating a modified hypothesis must accurately represent the observed experimental results.

Key Concept

Formulating and Modifying Hypotheses
Estimated Time:1m 30s
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