Question

Difficulty: HardDetermining Control Groups and Baseline Conditions

### Passage

An environmental scientist investigated the efficiency of sunflower plants (*Helianthus annuus*) in phytoremediation—the process of using living plants to remove heavy metals from contaminated soil. Sunflowers were grown in pots containing soil with a baseline lead (Pb2+Pb^{2+}) concentration of 500 mg/kg500\text{ mg/kg} under controlled greenhouse conditions. The scientist set up several experimental groups to evaluate how two soil amendments—biochar and arbuscular mycorrhizal fungi (AMF)—affect the rate of Pb2+Pb^{2+} uptake by the plants.

The experimental groups were designed as follows:
* Group A: Soil with 0 mg/kg0\text{ mg/kg} of Pb2+Pb^{2+}, no biochar, no AMF.
* Group B: Soil with 500 mg/kg500\text{ mg/kg} of Pb2+Pb^{2+}, no biochar, no AMF.
* Group C: Soil with 500 mg/kg500\text{ mg/kg} of Pb2+Pb^{2+}, biochar added (5% w/w5\%\text{ w/w}), no AMF.
* Group D: Soil with 500 mg/kg500\text{ mg/kg} of Pb2+Pb^{2+}, no biochar, AMF added.
* Group E: Soil with 500 mg/kg500\text{ mg/kg} of Pb2+Pb^{2+}, biochar added (5% w/w5\%\text{ w/w}), AMF added.

All groups were watered daily with 100 mL100\text{ mL} of distilled water and kept at a constant temperature of 24C24^\circ\text{C} under a 14-hour14\text{-hour} light/10-hour10\text{-hour} dark cycle. After 6 weeks6\text{ weeks}, the dry biomass of the plants and the concentration of Pb2+Pb^{2+} in the plant tissues were measured.

To evaluate the specific influence of each experimental variable, the scientist must compare the results of a test group to a corresponding control or baseline group. Match each of the following experimental objectives with the group that serves as the most appropriate control or baseline.

  • Determine the baseline growth of the plants in the absence of lead contamination.Group A (0 mg/kg0\text{ mg/kg} Pb2+Pb^{2+}, no biochar, no AMF)
  • Isolate the effect of the biochar amendment on lead accumulation in the absence of AMF.Group B (500 mg/kg500\text{ mg/kg} Pb2+Pb^{2+}, no biochar, no AMF)
  • Isolate the specific effect of biochar when AMF is already present in the soil.Group D (500 mg/kg500\text{ mg/kg} Pb2+Pb^{2+}, no biochar, AMF added)
  • Isolate the specific effect of AMF when biochar is already present in the soil.Group C (500 mg/kg500\text{ mg/kg} Pb2+Pb^{2+}, biochar added, no AMF)

Answer

To isolate a specific variable, the control group must keep all other variables identical to the test group except for the one being tested. The baseline growth of plants without contamination is established by Group A. The isolated effect of biochar in the absence of AMF is found by comparing Group C to Group B. The isolated effect of biochar in the presence of AMF is found by comparing Group E to Group D. The isolated effect of AMF in the presence of biochar is found by comparing Group E to Group C.
To isolate the effect of a single independent variable, you compare the experimental group (which contains the variable) to a control group that is identical in every way except it lacks that specific variable. Group A acts as the baseline for non-contaminated growth. Group B acts as the baseline for contaminated growth without amendments. Comparing Group E to Group D isolates the effect of biochar, since Group D already has AMF. Comparing Group E to Group C isolates the effect of AMF, since Group C already has biochar.

Step-by-Step Solution

1
Identify the independent variables in the experiment.
The independent variables are lead concentration (00 vs. 500 mg/kg500\text{ mg/kg}), biochar amendment (none vs. 5% w/w5\%\text{ w/w}), and AMF inoculation (none vs. added).
Understanding the variables is essential to determining which ones must be held constant to isolate the target effect.
2
Determine the control needed for baseline growth.
Group A has no lead, no biochar, and no AMF, which represents the background growth rate of sunflowers.
To see the effect of lead itself, plants must be grown without lead and without any remedial amendments.
3
Isolate biochar alone without AMF.
Comparing Group C (lead + biochar) to Group B (lead + no biochar) isolates biochar since AMF is absent in both.
Group B serves as the control because it has the same lead contamination level and lacks the biochar amendment.
4
Isolate biochar in the presence of AMF.
Comparing Group E (lead + biochar + AMF) to Group D (lead + no biochar + AMF) isolates biochar because AMF is held constant.
To see the effect of adding biochar when AMF is present, the control group must have AMF but not biochar.
5
Isolate AMF in the presence of biochar.
Comparing Group E (lead + biochar + AMF) to Group C (lead + biochar + no AMF) isolates AMF because biochar is held constant.
To see the effect of adding AMF when biochar is present, the control group must have biochar but not AMF.

Key Concept

Determining Control Groups and Baseline Conditions
Estimated Time:2m 0s
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