Question

Difficulty: EasyDesigning Follow-Up Experiments and Test Modifications

A student conducted an experiment to measure the rate of water loss from a certain plant species under different relative humidity levels. The experimental design is summarized in the table below:

TrialRelative Humidity (%)Temperature (C^\circ\text{C})Plant SpeciesExposure Time (hours)
12025Fern2
24025Fern2
36025Fern2
48025Fern2

Suppose the student wants to perform a follow-up experiment to determine how temperature affects the water loss of this same plant species. Which of the following modifications to the experimental design would best allow the student to isolate the effect of temperature?

  1. Vary the temperature across trials while keeping the relative humidity, plant species, and exposure time constant.Answer
  2. B
    Vary both the temperature and the relative humidity across trials while keeping the plant species and exposure time constant.
  3. C
    Vary the temperature and use a different plant species for each trial while keeping the relative humidity constant.
  4. D
    Vary the temperature across trials while increasing the exposure time for each increase in temperature.

Answer

Vary the temperature across trials while keeping the relative humidity, plant species, and exposure time constant.
To investigate the effect of a new independent variable (temperature), that variable must be varied while holding all other variables constant. The correct option describes changing the temperature across trials while maintaining constant relative humidity, plant species, and exposure time, which successfully isolates the effect of temperature.

Step-by-Step Solution

1
Identify the goal of the follow-up experiment.
The goal is to determine the specific effect of temperature on water loss (transpiration).
This establishes temperature as the new independent variable.
2
Apply the rule of experimental control.
To isolate the effect of temperature, only temperature should vary, while all other potential independent variables (such as relative humidity, plant species, and exposure time) must remain constant.
Varying multiple factors simultaneously introduces confounding variables, which prevents a clear conclusion about which variable caused the change.
3
Evaluate the choices to find the one that varies only temperature.
The option to vary the temperature while holding relative humidity, plant species, and exposure time constant correctly isolates the variable of interest.
This aligns with proper scientific method and experimental design.

Key Concept

Scientific Control of Variables in Follow-Up Experiments
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