An audio guide provides the following calibration protocol for an automated hydroponic dosing unit:
"To properly calibrate the automated delivery system, begin by pre-rinsing the mixing chamber with 50 liters of deionized water at a flow rate of 10 liters per minute. Next, inject the concentrated macro-nutrient formula until the electrical conductivity reaches exactly 1.8 millisiemens per centimeter. Following this, activate the aeration pump for 15 minutes to stabilize the dissolved oxygen above 6.5 milligrams per liter. Finally, adjust the acid dosing regulator to achieve a steady pH balance between 5.8 and 6.0 before opening the main irrigation line."
Based on the audio transcript, arrange the calibration steps in the correct chronological order from first to last.
- 1Flush the mixing reservoir with 50 liters of deionized water at 10 liters per minute.
- 2Add concentrated macro-nutrients until reaching an electrical conductivity of 1.8 mS/cm.
- 3Run aeration for 15 minutes to raise dissolved oxygen above 6.5 mg/L.
- 4Regulate acid injection to stabilize the pH between 5.8 and 6.0 before irrigation.