Passage:
In 1898, Dutch microbiologist Martinus Beijerinck published findings demonstrating that the agent responsible for tobacco mosaic disease was fundamentally distinct from bacteria. Unlike bacterial pathogens, which could be removed from liquid suspension by filtration through porcelain Chamberland filters, the mosaic agent passed unimpeded through the fine pores. Furthermore, Beijerinck observed that while the agent failed to multiply in nutrient broth or cell-free culture media, it proliferated readily when introduced to living, actively growing plant tissues. To describe this novel entity, he coined the term 'contagium vivum fluidum' (contagious living fluid). Although Beijerinck initially hypothesized that the agent was liquid in nature rather than particulate, his crucial insight lay in establishing its absolute dependence on host cellular machinery for replication—a foundational distinction that separated viruses from known cellular microorganisms.
According to the passage, which of the following statements regarding Martinus Beijerinck's research on the tobacco mosaic agent are explicitly supported?
Select all that apply.
- The tobacco mosaic agent was capable of passing through porcelain Chamberland filters.Cevap
- BThe mosaic agent multiplied efficiently in nutrient broth solutions without host tissue.
- The mosaic agent required living, actively growing host tissue in order to replicate.Cevap