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Zorluk: OrtaSublimation, Magnetization, and Centrifugation

A clinical laboratory technician needs to rapidly separate dense cellular components, such as red blood cells, from liquid plasma in a blood sample. Which technique utilizes high-speed rotation to achieve this separation based on density differences?

  1. CentrifugationCevap
  2. B
    Sublimation
  3. C
    Magnetization
  4. D
    Fractional distillation

Cevap

Centrifugation is the correct technique for separating suspended cellular components from blood plasma based on density differences.
Centrifugation relies on spinning a fluid mixture at high speeds. The resulting outward rotational force forces denser solid components (such as red blood cells) to settle to the bottom of the tube faster than natural gravity would allow, separating them from the lighter liquid supernatant (plasma).

Adım Adım Çözüm

1
Identify the physical state and properties of the components in whole blood.
Blood is a liquid suspension consisting of denser solid/cellular components (red and white blood cells, platelets) suspended in liquid plasma.
Choosing an effective separation technique requires analyzing differences in physical properties such as particle size, state of matter, magnetic property, or density.
2
Evaluate the mechanism of centrifugation.
Centrifugation spins the mixture at extremely high speeds in a centrifuge tube.
High-speed rotation creates outward centrifugal force, accelerating the sedimentation of denser suspended particles to the bottom (forming a pellet) while the lighter liquid remains as a clear supernatant layer.
3
Compare centrifugation against alternative separation techniques.
Sublimation requires solid-to-gas phase transitions, magnetization requires magnetic components, and distillation requires thermal boiling of liquid mixtures. None of these are suitable for delicate cell suspensions.
Centrifugation is uniquely suited for separating colloidal or fine suspensions without thermal degradation.

Anahtar Kavram

Centrifugation uses centrifugal force produced by high-speed rotation to separate suspended solids from liquids or immiscible liquids of different densities.
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Konular

Sublimation, Magnetization, and Centrifugation