Question

Difficulty: HardDesigning Follow-Up Experiments and Test Modifications

A scientist conducted two experiments to study soil respiration (measured as the rate of carbon dioxide release, RsR_s, in g CO2/m2/day\text{g CO}_2/\text{m}^2/\text{day}) from a forest soil sample under different conditions.

ExperimentSoil Temperature (C^\circ\text{C})Soil Moisture Content (SMC)Observed Trend in RsR_s
120C20^\circ\text{C} (constant)Varied (10%10\%, 20%20\%, 30%30\%)RsR_s increases as SMC increases
2Varied (15C15^\circ\text{C}, 25C25^\circ\text{C}, 35C35^\circ\text{C})20%20\% (constant)RsR_s increases as temperature increases

Suppose a scientist wants to determine if the positive relationship between SMC and RsR_s observed at 20C20^\circ\text{C} remains positive at a near-freezing temperature of 2C2^\circ\text{C}. Which of the following modifications to the experimental design would best allow the scientist to test this hypothesis?

  1. Measure RsR_s of soil samples at a constant temperature of 2C2^\circ\text{C} while varying the SMC at 10%10\%, 20%20\%, and 30%30\%.Answer
  2. B
    Measure RsR_s of soil samples as the temperature is decreased from 20C20^\circ\text{C} to 2C2^\circ\text{C} while keeping the SMC constant at 20%20\%.
  3. C
    Measure RsR_s of soil samples at temperatures of 2C2^\circ\text{C}, 10C10^\circ\text{C}, and 20C20^\circ\text{C} while allowing the SMC to vary freely for each sample.
  4. D
    Measure the rate of water evaporation from the soil samples at 2C2^\circ\text{C} as the SMC is varied at 10%10\%, 20%20\%, and 30%30\%.

Answer

Measure RsR_s of soil samples at a constant temperature of 2C2^\circ\text{C} while varying the SMC at 10%10\%, 20%20\%, and 30%30\%.
To determine if the positive relationship between soil moisture content (SMC) and soil respiration rate (RsR_s) is preserved at a new constant temperature (2C2^\circ\text{C}), the scientist must isolate SMC as the independent variable. This is accomplished by holding the temperature constant at 2C2^\circ\text{C} and measuring RsR_s across the same range of SMC values (10%10\%, 20%20\%, and 30%30\%) used in the original experiment.

Step-by-Step Solution

1
Identify the goal of the proposed follow-up experiment.
The goal is to determine if the positive relationship between SMC and RsR_s holds at 2C2^\circ\text{C}.
Understanding the hypothesis is necessary to determine which variables must be manipulated and measured.
2
Identify the independent and dependent variables required to test the hypothesis.
The independent variable must be SMC (varied at 10%10\%, 20%20\%, and 30%30\%) and the dependent variable must be RsR_s (soil respiration rate).
To see if the relationship between moisture and respiration is altered, moisture must be varied while measuring respiration.
3
Identify the necessary control variables.
The temperature must be held constant at the new temperature of interest (2C2^\circ\text{C}).
If temperature is not held constant at 2C2^\circ\text{C}, the effect of temperature will confound the results.
4
Select the option that matches these design criteria.
Varying SMC while maintaining a constant temperature of 2C2^\circ\text{C} and measuring RsR_s is the correct experimental design.
This setup isolates the effect of SMC at the specific temperature of 2C2^\circ\text{C} without introducing confounding variables.

Key Concept

To test the relationship between an independent variable and a dependent variable under a new constant condition, the independent variable must be varied while keeping all other conditions constant.
Estimated Time:2m 0s
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