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Zorluk: KolayPaper and Column Chromatography

During the chromatographic analysis of an ink sample, a component spot moves a distance of 3.0 cm3.0\text{ cm} from the origin line while the solvent front advances 10.0 cm10.0\text{ cm}. What is the retardation factor (RfR_f) for this component?

  1. 0.300.30Cevap
  2. B
    3.333.33
  3. C
    0.700.70
  4. D
    3.003.00

Cevap

The retardation factor (RfR_f) for the component is 0.300.30.
The retardation factor (RfR_f) is calculated by dividing the distance moved by the solute spot (3.0 cm3.0\text{ cm}) by the distance moved by the solvent front (10.0 cm10.0\text{ cm}), giving 0.300.30.

Adım Adım Çözüm

1
Identify the distance traveled by the component spot and the distance traveled by the solvent front.
Distance moved by solute spot = 3.0 cm3.0\text{ cm}; Distance moved by solvent front = 10.0 cm10.0\text{ cm}.
These values are required to calculate the retardation factor.
2
Apply the retardation factor formula: Rf=Distance moved by solute spotDistance moved by solvent frontR_f = \frac{\text{Distance moved by solute spot}}{\text{Distance moved by solvent front}}.
Rf=3.0 cm10.0 cm=0.30R_f = \frac{3.0\text{ cm}}{10.0\text{ cm}} = 0.30.
The RfR_f value represents the relative movement of the solute with respect to the mobile phase.

Anahtar Kavram

Calculation of Retardation Factor (Rf) in paper chromatography
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Konular

Paper and Column Chromatography

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