Arrange the following sequential steps of a paper chromatography experiment in the correct procedural order from start to finish.
- 1Draw a light baseline using a lead pencil about from the bottom edge of the filter paper strip.
- 2Apply a small, concentrated spot of the sample mixture onto the baseline and allow it to dry.
- 3Suspend the paper strip in a sealed chamber, ensuring the solvent level remains strictly below the baseline.
- 4Remove the strip when the mobile phase nears the top and immediately pencil-mark the solvent front.
Cevap
The correct procedural order is: (1) Draw a light baseline using a lead pencil about from the bottom edge of the filter paper strip, (2) Apply a small, concentrated spot of the sample mixture onto the baseline and allow it to dry, (3) Suspend the paper strip in a sealed chamber, ensuring the solvent level remains strictly below the baseline, and (4) Remove the strip when the mobile phase nears the top and immediately pencil-mark the solvent front.
The correct sequence follows standard analytical chromatography protocol: first, a graphite pencil baseline is drawn at the base of the paper strip. Next, the mixture is spotted onto this origin baseline. The paper is then placed into the developing tank with the mobile phase level kept below the baseline to allow upward capillary migration. Finally, the paper is removed before the solvent reaches the top edge, and the solvent front is marked immediately for accurate distance measurements.
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Anahtar Kavram
Laboratory procedural steps and operational principles of paper chromatography